GRUP DE RECERCA EN  FISIOLOGIA I NUTRICIÓ EXPERIMENTAL

Universitat de Barcelona (Barcelona)

Directora: Joana Maria Planas Rosselló, Catedràtica d’Universitat

A/e de contacte: jmplanas@ub.edu

Telèfon de contacte: +34) 934 02 45 05

Actualització fitxa tècnica del grup: novembre 2018

MEMBRES DEL GRUP INVESTIGADOR

Joana Maria Planas Rosselló, Catedràtica de Universitat, Universitat de Barcelona. a/e: jmplanas@ub.edu

Miquel Moretó Pedragosa, Catedràtic de Universitat, Universitat de Barcelona. a/e: mmoreto@ub.edu

Concepció Amat Mirallés, Professora Titular de Universitat, Universitat de Barcelona. a/e: camat@ub.edu

Maria Emilia Juan Olivé, Professora Agregada, Universitat de Barcelona. a/e: mejuan@ub.edu

Anna Pérez Bosque, Professora Agregada Interina, Universitat de Barcelona. a/e: anna.perez@ub.edu

Maria Lluïsa Miró, Professora Associada, Universitat de Barcelona. a/e: lluisa.miro@ub.edu

Maria Carme Villà Blasco, Professora Associada, Universitat de Barcelona. a/e: mcvilla@ub.edu

Richard J. Naftalin, Professor Emèrit, Universitat de Barcelona. a/e: richard.naftalin@kcl.ac.uk

Rocio Moreno González, Investigadora predoctoral, Universitat de Barcelona. a/e: rociomorenogonzalez@ub.edu

Tàlia Franco Àvila, Investigadora predoctoral, Universitat de Barcelona. a/e: talia.franco@ajanut.org

Ivana Kundisova, Investigadora predoctoral, Universitat de Barcelona. a/e: ivana.kundisova@gmail.com

Cristina Rossell Cardona, Investigadora predoctoral, Universitat de Barcelona. a/e: cristina.rossell@ub.edu

Paula Barlabé Ginesta, Tècnic suport a la recerca, Universitat de Barcelona. a/e: paula.barlabe@ub.edu

ACTIVITATS I CAPACITATS DEL GRUP DE RECERCA

El Grup porta a terme recerca subvencionada per recursos públics i recerca mitjançant contractes amb empreses, orientada al descobriment de propietats o activitats biològiques de components dels aliments o de suplements dietètics. En el marc d’aquesta temàtica, el grup de recerca desenvolupa línies que permeten conèixer el mecanisme d’absorció intestinal així com la biodisponibilitat de compostos bioactius de la dieta, que s’estudien tant de forma individual, com dins de l’aliment. Aquesta recerca permet conèixer si els compostos poden arribar a la sang en concentracions suficients per realitzar els seus efectes terapèutics, conèixer quin és el seu metabolisme i/o eliminació així com els òrgans diana. A més també es realitzen estudis de toxicitat per poder assegurar que són compostos segurs pels seu possible us com a nutracèutics. Un altre aspecte important de la recerca del grup, és l’avaluació dels efectes dels ingredients funcionals sobre la funció intestinal, tant en animals sans, com amb patologies d’alt efecte social, com ara, la malaltia inflamatòria intestinal (IBD), l’envelliment, hipertensió i diabetis.

LÍNIES DE RECERCA

Línia: Fisiologia i fisiopatologia nutricional. Estudi de la biofuncionalitat dels aliments i ingredients funcionals que engloben l’avaluació de l’absorció i el metabolisme intestinal, la biodisponibilitat oral, així com l’estudi de la toxicitat oral que acompanyen els dels efectes biològics en diferents models animals d’hipertensió, diabetis i càncer de colon.
Investigador principal: Joana M. Planas

Línia: Fisiologia digestiva i adaptacions nutricionals. Estudi experimental de la regulació dels canvis estructurals i funcionals que es produeixen en el còlon distal en el decurs de l’adaptació a dietes amb baix contingut en sodi. Paper de les hormones aldosterona, angiotensina II i vasopressina. Interaccions entre miofibroblasts i enteròcits de la cripta.
Investigador principal: Miquel Moretó

Línia: Permeabilitat intestinal i transport de nutrients. Absorció d’electròlits i no electròlits per l’epiteli intestinal en models experimentals in vivo i in vitro. Estudi de la permeabilitat paracel·lular i dels factors que la modifiquen. Estudi dels canvis funcionals de l’intestí com a resposta als nutracèutics.
Investigador principal: Concepció Amat

Línia: Models d’inflamació mucosal i teràpia nutricional. Estudi i caracterització de diferents models d’inflamació mucosal (pulmonar, intestinal). Possible aplicació d’ingredients funcionals com a teràpia nutricional.
Investigador principal: Anna Pérez Bosque

Línia: Aplicació de tècniques de cromatografia a la determinació d’ingredients funcionals i els seus metabòlits. Anàlisi de compostos bioactius de la dieta en mostres biològiques (plasma, orina i teixits) mitjançant l’ús de cromatografia líquida acoblada a espectrometria de masses (triple quadrupol/QTRAP), i espectrometria de masses d’alta resolució (Orbitrap).
Investigador principal: M. Emília Juan

MILLORS PUBLICACIONS DEL GRUP (DARRERS 5 ANYS)

Giménez E, Juan ME, Calvo-Melià S, Planas JM.
A sensitive liquid chromatography-mass spectrometry method for the simultaneous determination in plasma of pentacyclic triterpenes of Olea europaea L.
Food Chem. 2017; 229: 229:534-541.
PMID: 28372212

ABSTRACT

Table olives are especially rich in pentacyclic triterpenic compounds, which exert several biological activities. A crucial step in order to know if these compounds could contribute to the beneficial and healthy properties of this food is their measurement in blood. Therefore, the present study describes a simple and accurate liquid-liquid extraction followed by LC-QqQ-MS analysis for the simultaneous determination of the main pentacyclic triterpenes from Olea europaea L. in rat plasma. The method was validated by the analysis of blank plasma samples spiked with pure compounds, obtaining a linear correlation, adequate sensitivity with a limit of quantification ranging from 1nM for maslinic acid to 10nM for uvaol. Precision and accuracy were lower than 10% in all cases and recoveries were between 95 and 104%. The oral administration of olives to rats and its determination in plasma verified that the established methodology is appropriate for bioavailability studies.

Miró L, Garcia-Just A, Amat C, Polo J, Moretó M, Pérez-Bosque A.
Dietary Animal Plasma Proteins Improve the Intestinal Immune Response in Senescent Mice.
Nutrients. 2017. 9(12). pii: E1346.
PMID: 29232896

ABSTRACT

Increased life expectancy has promoted research on healthy aging. Aging is accompanied by increased non-specific immune activation (inflammaging) which favors the appearance of several disorders. Here, we study whether dietary supplementation with spray-dried animal plasma (SDP), which has been shown to reduce the activation of gut-associated lymphoid tissue (GALT) in rodents challenged by S. aureus enterotoxin B (SEB), and can also prevent the effects of aging on immune system homeostasis. We first characterized GALT in a mouse model of accelerated senescence (SAMP8) at different ages (compared to mice resistant to accelerated senescence; SAMR1). Second, we analyzed the SDP effects on GALT response to an SEB challenge in SAMP8 mice. In GALT characterization, aging increased the cell number and the percentage of activated Th lymphocytes in mesenteric lymph nodes and Peyer’s patches (all, p < 0.05), as well as the expression of IL-6 and TNF-α in intestinal mucosa (both, p < 0.05). With respect to GALT response to the SEB challenge, young mice showed increased expression of intestinal IL-6 and TNF-α, as well as lymphocyte recruitment and activation (all, p < 0.05). However, the immune response of senescent mice to the SEB challenge was weak, since SEB did not change cell recruitment or the percentage of activated Th lymphocytes. Mice supplemented with SDP showed improved capacity to respond to the SEB challenge, similar to the response of the young mice. These results indicate that senescent mice have an impaired mucosal immune response characterized by unspecific GALT activation and a weak specific immune response. SDP supplementation reduces non-specific basal immune activation, allowing for the generation of specific responses.

Lozano-Mena G, Sánchez-González M, Parra A, Juan ME, Planas JM.
Identification of gut-derived metabolites of maslinic acid, a bioactive compound from Olea europaea L.
Mol Nutr Food Res. 60(9):2053-64.
PMID: 27144997

ABSTRACT

SCOPE: Maslinic acid has been described to exert a chemopreventive activity in colon cancer. Hereby, we determined maslinic acid and its metabolites in the rat intestine previous oral administration as a first step in elucidating whether this triterpene might be used as a nutraceutical.
METHODS AND RESULTS: Maslinic acid was orally administered at 1, 2, and 5 mg/kg to male Sprague-Dawley for 2 days. At 24 h after the last administration, the content of the duodenum and jejunum, ileum, cecum, and colon was collected and extracted with methanol 80% prior to LC-APCI-MS analysis. The developed method was validated providing suitable sensitivity (LOQ of 5 nM), good recovery (97.8 ± 3.6%), linear correlation, and appropriate precision (< 9%). Maslinic acid was detected in all the segments with higher concentrations in the distal part of the intestine. LC-APCI-LTQ-ORBITRAP-MS allowed the identification of 11 gut-derived metabolites that were formed by mono-, dihydroxylation, and dehydrogenation reactions.
CONCLUSION: Maslinic acid undergoes phase I reactions resulting in a majority of monohydroxylated metabolites without the presence of phase II derivatives. The high concentration of maslinic acid achieved in the intestine suggests that it could exert a beneficial effect in the prevention of colon cancer.

Pérez-Bosque A, Miró L, Amat C, Polo J, Moretó M.
The Anti-Inflammatory Effect of Spray-Dried Plasma Is Mediated by a Reduction in Mucosal Lymphocyte Activation and Infiltration in a Mouse Model of Intestinal Inflammation.
Nutrients. 2016. 8(10). pii: E657.
PMID: 27782068

ABSTRACT

Spray-dried preparations from porcine and bovine plasma can alleviate mucosal inflammation in experimental models and improve symptoms in patients with enteropathy. In rodents, dietary supplementation with porcine spray-dried plasma (SDP) attenuates intestinal inflammation and improves the epithelial barrier function during intestinal inflammation induced by Staphylococcus aureus enterotoxin B (SEB). The aim of this study was to discern the molecular mechanisms involved in the anti-inflammatory effects of SDP. Male C57BL/6 mice were fed with 8% SDP or control diet (based on milk proteins) for two weeks, from weaning until day 33. On day 32, the mice were given a SEB dose (i.p., 25 µg/mouse) or vehicle. SEB administration increased cell recruitment to mesenteric lymph nodes and the percentage of activated Th lymphocytes and SDP prevented these effects). SDP supplementation increased the expression of interleukin 10 (IL-10) or transforming growth factor- β (TGF-β) compared to the SEB group. The SEB challenge increased six-fold the expression of mucosal addressin cell adhesion molecule 1 (MAdCAM-1) and intercellular adhesion molecule 1 (ICAM-1); and these effects were attenuated by SDP supplementation. SEB also augmented NF-κB phosphorylation, an effect that was prevented by dietary SDP. Our results indicate that the anti-inflammatory effects of SDP involve the regulation of transcription factors and adhesion molecules that reduce intestinal cell infiltration and the degree of the inflammatory response.

Pérez-Bosque A, Miró L, Maijó M, Polo J, Campbell JM, Russell L, Crenshaw JD, Weaver E, Moretó M.
Oral Serum-Derived Bovine Immunoglobulin/Protein Isolate Has Immunomodulatory Effects on the Colon of Mice that Spontaneously Develop Colitis.
PLoS One. 2016. 11(5):e0154823.
PMID: 27139220

ABSTRACT

Dietary immunoglobulin concentrates prepared from animal plasma can modulate the immune response of gut-associated lymphoid tissue (GALT). Previous studies have revealed that supplementation with serum-derived bovine immunoglobulin/protein isolate (SBI) ameliorates colonic barrier alterations in the mdr1a-/- genetic mouse model of IBD. Here, we examine the effects of SBI on mucosal inflammation in mdr1a-/- mice that spontaneously develop colitis. Wild type (WT) mice and mice lacking the mdr1a gene (KO) were fed diets supplemented with either SBI (2% w/w) or milk proteins (Control diet), from day 21 (weaning) until day 56. Leucocytes in mesenteric lymph nodes (MLN) and in lamina propria were determined, as was mucosal cytokine production. Neutrophil recruitment and activation in MLN and lamina propria of KO mice were increased, but were significantly reduced in both by SBI supplementation (p < 0.05). The increased neutrophil recruitment and activation observed in KO mice correlated with increased colon oxidative stress (p < 0.05) and SBI supplementation reduced this variable (p < 0.05). The Tact/Treg lymphocyte ratios in MLN and lamina propria were also increased in KO animals, but SBI prevented these changes (both p < 0.05). In the colon of KO mice, there was an increased production of mucosal pro-inflammatory cytokines such as IL-2 (2-fold), IL-6 (26-fold) and IL-17 (19-fold), and of chemokines MIP-1β (4.5-fold) and MCP-1 (7.2-fold). These effects were significantly prevented by SBI (p < 0.05). SBI also significantly increased TGF-β secretion in the colon mucosa, suggesting a role of this anti-inflammatory cytokine in the modulation of GALT and the reduction of the severity of the inflammatory response during the onset of colitis.

INSTITUCIONS QUE RECONEIXEN AL GRUP DE RECERCA

Generalitat de Catalunya
Programa d’ajuts SGR a grups consolidats de recerca, número de referència: 201-SGR-945

Universitat de Barcelona
Institut de recerca en Nutrició i Seguretat Alimentària