GRUP DE RECERCA ANTIOXIDANTS NATURALS: POLIFENOLS

Universitat de Barcelona (Barcelona)Director: Rosa Maria Lamuela-Raventós
Adreça mail de contacte del grup de Recerca: lamuela@ub.edu
Telèfon de contacte: (+34) 934 03 48 43
Web: http://www.polyphenolresearch.com/

Actualització fitxa tècnica del grup: juliol 2017

MEMBRES DEL GRUP INVESTIGADOR

Rosa María Lamuela Raventós; Professora Titular, Universitat de Barcelona
a/e: lamuela@ub.edu

Anna Tresserra Rimbau; Investigador Postdoctoral, CIBEROBN
a/e: annatresserra@ub.edu

Paola Quifer Rada; Investigador Postdoctoral, Universitat de Barcelona
a/e: paolaquifer@ub.edu

José Fernando Rinaldi de Alvarenga; Investigador Predoctoral, Universitat de Barcelona
a/e: jrinaldi@ub.edu

Gemma Sasot Flix; Investigador Predoctoral, CIBEROBN
a/e: g.sasotflix@ub.edu

Sara Hurtado Barroso; Investigador Predoctoral, Universitat de Barcelona
a/e: sara.hurtado_17@ub.edu

Simeón Ruiz Martí; Técnic, CIBEROBN
a/e: simeonruiz@ub.edu

ACTIVITATS I CAPACITATS DEL GRUP DE RECERCA

Les nostres línies de recerca es centren en els compostos bioactius i la seva biodisponibilitat i efectes en la salut de les persones i els animals. Treballem amb un ampli ventall d’aliments, pels que identifiquem i quantifiquem polifenols i carotenoides, a més a més utilitzem les tècniques analítiques més modernes en el camp de la espectrometria de masses per a identificar i quantificar components bioactius i els seus metabòlits en mostres biològiques d’estudis clínics. El nostre grup de recerca, ha estat involucrat en estudis nacionals i internacionals com l’estudi PREDIMED i l’estudi EPIC. Mantenim contacte i col·laborem amb algunes universitats internacionals de primer nivell com Harvard School of Public Health, Boston i UC Davis, California entre d’altres. Hem estat grup consolidat de recerca per la Generalitat de Catalunya a les convocatòries SGR de l’any 2014 i 2009. Pertanyem al XARTA (Xarxa de Referència en Tecnologia dels Aliments de la Generalitat de Catalunya), a l’INSA-UB (Institut de Recerca en Nutrició i Seguretat Alimentària de la Universitat de Barcelona) i al CIBEROBN (Centro de Investigación Biomédica en Red-Fisiopatología de la Obesidad y Nutrición CB12/03/30020). Tot això indica que som un grup ben preparat i capaç de realitzar estudis clínics i epidemiològics tot seguint els estàndards d’excel·lència de les institucions que ens reconeixen. El nostre objectiu es fer recerca bàsica i aplicada del més alt nivell i preparar personal crític i autònom per a la recerca internacional i assolir els requeriments de la industria produint impacte en la societat.

LÍNIES DE RECERCA

Línia: Anàlisi de polifenols en aliments (vins, oli, cacau, tomàquet, cervesa…)
Investigador principal: Rosa María Lamuela Raventós

Línia: Estudis Clínics i Epidemiològics
Investigador principal: Rosa María Lamuela Raventós

Línia: Anàlisi de polifenols en mostres biològiques
Investigador principal: Rosa María Lamuela Raventós

Línia: Anàlisi de polifenols de raïm i vins en restes arqueològiques
Investigador principal: Rosa María Lamuela Raventós

Línia: Factors que modifiquen la presència dels components bioactius i la seva efectivitat.
Investigador principal: Rosa María Lamuela Raventós

MILLORS PUBLICACIONS DEL GRUP (2015-2017)

Quifer-Rada P, Choy YY, Calvert CC, Waterhouse AL, Lamuela-Raventos RM.
Use of metabolomics and lipidomics to evaluate the hypocholestreolemic effect of Proanthocyanidins from grape seed in a pig model.
Mol Nutr Food Res. 2016; Oct;60(10): 2219-2227.
PMID: 27240545

SCOPE: This work aims to evaluate changes in the fecal metabolomic profile due to grape seed extract (GSE) intake by untargeted and targeted analysis using high resolution mass spectrometry in conjunction with multivariate statistics.
METHODS AND RESULTS: An intervention study with six crossbred female pigs was performed. The pigs followed a standard diet for 3 days, then they were fed with a supplemented diet containing 1% (w/w) of MegaNatural® Gold grape seed extract for 6 days. Fresh pig fecal samples were collected daily. A combination of untargeted high resolution mass spectrometry, multivariate analysis (PLS-DA), data-dependent MS/MS scan, and accurate mass database matching was used to measure the effect of the treatment on fecal composition. The resultant PLS-DA models showed a good discrimination among classes with great robustness and predictability. A total of 14 metabolites related to the GSE consumption were identified including biliary acid, dicarboxylic fatty acid, cholesterol metabolites, purine metabolites, and eicosanoid metabolites among others. Moreover, targeted metabolomics using GC-MS showed that cholesterol and its metabolites fecal excretion was increased due to the proanthocyanidins from grape seed extract.
CONCLUSION: The results show that oligomeric procyanidins from GSE modifies bile acid and steroid excretion, which could exert a hypocholesterolemic effect.

Lamuela-Raventos RM, Quifer-Rada P.
Effect of dietary polyphenols on cardiovascular risk.
Heart. 2016; Sep 1;102(17):1340-1.
PMID: 27325587

Vallverdú-Queralt A, Boix N, Piqué E, Gómez-Catalan J, Medina-Remon A, Sasot G, Mercader-Martí M, Llobet JM, Lamuela-Raventos RM.
Identification of phenolic compounds in red wine extract samples and zebrafish embryos by HPLC-ESI-LTQ-Orbitrap-MS.
Food Chem. 2015; Aug 15;181:146-51.
PMID: 25794733
Abstract: The zebrafish embryo is a highly interesting biological model with applications in different scientific fields, such as biomedicine, pharmacology and toxicology. In this study, we used liquid chromatography/electrospray ionisation-linear ion trap quadrupole-Orbitrap-mass spectrometry (HPLC/ESI-LTQ-Orbitrap-MS) to identify the polyphenol compounds in a red wine extract and zebrafish embryos. Phenolic compounds and anthocyanin metabolites were determined in zebrafish embryos previously exposed to the red wine extract. Compounds were identified by injection in a high-resolution system (LTQ-Orbitrap) using accurate mass measurements in MS, MS(2) and MS(3) modes. To our knowledge, this research constitutes the first comprehensive identification of phenolic compounds in zebrafish by HPLC coupled to high-resolution mass spectrometry.

Quifer-Rada P, Vallverdú-Queralt A, Martínez-Huélamo M, Chiva-Blanch G, Jáuregui O, Estruch R, Lamuela-Raventós R.
A comprehensive characterisation of beer polyphenols by high resolution mass spectrometry (LC-ESI-LTQ-Orbitrap-MS).
Food Chem. 2015; Feb 15;169:336-43.
PMID: 25236235
Abstract: Beer is the second most consumed alcoholic beverage in Europe and shown by the European Prospective Investigation into Cancer and Nutrition cohort study to be the main food contributor to hydroxybenzoic acid intake. About 70-80% of the total polyphenol content in beer comes from malt, and the remaining 30-20% from hops. In this work, liquid chromatography coupled with an electrospray ionization hybrid linear ion trap quadrupole Orbitrap mass spectrometry technique has been used for an accurate identification of beer polyphenols. 47 phenolic compounds were identified using high mass accuracy and confirmed by MS(2) experiments, including simple phenolic acids, hydroxycinnamoylquinics, flavanols, flavonols, flavones, alkylmethoxyphenols, alpha- and iso-alpha-acids, hydroxyphenylacetic acids and prenylflavonoids. As far as we know, 7 of these compounds have been recognised in beer for the first time: feruloylquinic acid, caffeic acid-O-hexoside, coumaric acid-O-hexoside, sinapic acid-O-hexoside, catechin-O-dihexoside, kaempferol-O-hexoside, and apigenin-C-hexoside-pentoside.

Martínez-Huélamo M, Vallverdú-Queralt A, Di Lecce G, Valderas-Martínez P, Tulipani S, Jáuregui O, Escribano-Ferrer E, Estruch R, Illan M, Lamuela-Raventós RM.
Bioavailability of tomato polyphenols is enhanced by processing and fat addition: Evidence from a randomized feeding trial.
Mol Nutr Food Res. 2016; Jul;60(7):1578-89.
PMID: 26887966

SCOPE: Tomato contains a variety of phenolics associated with health-promoting properties. However, the effects of processing and the addition of oil during tomato sauce preparation on microbial metabolism of phenolics in the small intestine are still unclear.
METHODS AND RESULTS: An open, controlled, randomized, and crossover feeding trial with 40 healthy volunteers was carried out to analyze the metabolites in plasma and urine after the consumption of tomato and tomato sauces, with tomato sauce enriched with refined olive oil (ROOE) and without refined olive oil (oil-free: OF). Ten phenolics in plasma and 93 metabolites in urine were quantified. Processing tomatoes into sauce enhanced the bioavailability of flavanones, flavanols, and some hydroxycinnamic acids, as reflected by the increase in the area under the plasma concentration versus time curve. An increase in their plasma half-life was also observed, particularly after ingestion of ROOE, possibly favored by enterohepatic circulation. A wide variety of gut microbial metabolites was also detected, namely flavanones, hydroxycinnamic acids, flavonols, hydroxyphenylpropanoic acids, hydroxyphenylacetic acids, and hydroxybenzoic acids.
CONCLUSIONS: Flavanones and flavonols in ROOE presented higher bioavailability, suggesting that the processing undergone by the raw tomato improved their absorption.

INSTITUCIONS QUE RECONEIXEN AL GRUP DE RECERCA

AGAUR

Instituto de Salud Carlos III

Universitat de Barcelona